Polymerase chain reaction (PCR) is a technique used in molecular biology
to make millions of copies of DNA. PCR is now a common and used in
clinical and research laboratories for a broad variety of applications.
Real-time PCR monitors the process of amplification in real time using
fluorescence; not only at the end of the reaction as occurs in conventional PCR.
Digital PCR is a quantitative method that provides a sensitive way of measuring
the amount of DNA in a sample. Photomultiplier tube (PMT), SiPM(MPPC) and
CMOS camera are mainly considered for the fluorescence detection system

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